Polymerase Chain Reaction
"Polymerase Chain Reaction" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus,
MeSH (Medical Subject Headings). Descriptors are arranged in a hierarchical structure,
which enables searching at various levels of specificity.
In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.
| Descriptor ID |
D016133
|
| MeSH Number(s) |
E05.393.620.500
|
| Concept/Terms |
Polymerase Chain Reaction- Polymerase Chain Reaction
- Polymerase Chain Reactions
- Reaction, Polymerase Chain
- Reactions, Polymerase Chain
- PCR
Inverse PCR- Inverse PCR
- PCR, Inverse
- Inverse Polymerase Chain Reaction
Anchored PCR- Anchored PCR
- PCR, Anchored
- Anchored Polymerase Chain Reaction
|
Below are MeSH descriptors whose meaning is more general than "Polymerase Chain Reaction".
Below are MeSH descriptors whose meaning is more specific than "Polymerase Chain Reaction".
This graph shows the total number of publications written about "Polymerase Chain Reaction" by people in this website by year, and whether "Polymerase Chain Reaction" was a major or minor topic of these publications.
View timeline visualization
| Year | Major Topic | Minor Topic | Total |
|---|
| 1995 | 27 | 216 | 243 |
| 1996 | 18 | 225 | 243 |
| 1997 | 29 | 240 | 269 |
| 1998 | 17 | 231 | 248 |
| 1999 | 19 | 141 | 160 |
| 2000 | 19 | 137 | 156 |
| 2001 | 13 | 164 | 177 |
| 2002 | 20 | 170 | 190 |
| 2003 | 22 | 166 | 188 |
| 2004 | 22 | 220 | 242 |
| 2005 | 26 | 208 | 234 |
| 2006 | 20 | 179 | 199 |
| 2007 | 24 | 164 | 188 |
| 2008 | 23 | 180 | 203 |
| 2009 | 27 | 201 | 228 |
| 2010 | 36 | 223 | 259 |
| 2011 | 15 | 205 | 220 |
| 2012 | 15 | 162 | 177 |
| 2013 | 16 | 145 | 161 |
| 2014 | 23 | 117 | 140 |
| 2015 | 9 | 96 | 105 |
| 2016 | 17 | 116 | 133 |
| 2017 | 21 | 84 | 105 |
| 2018 | 8 | 60 | 68 |
| 2019 | 9 | 43 | 52 |
| 2020 | 10 | 41 | 51 |
| 2021 | 3 | 49 | 52 |
| 2022 | 2 | 21 | 23 |
| 2023 | 0 | 17 | 17 |
| 2024 | 3 | 11 | 14 |
| 2025 | 1 | 0 | 1 |
Below are the most recent publications written about "Polymerase Chain Reaction" by people in Profiles.
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Detection of Salmonella spp. in pooled environmental samples from an equine veterinary hospital using a novel point-of-care PCR assay. J Equine Vet Sci. 2025 Mar; 146:105376.
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Deep sequencing as a diagnostic tool in patients with suspected primary vitreoretinal lymphoma. Br J Ophthalmol. 2024 Dec 17; 109(1):70-75.
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Evaluation of 16s Long Read Metabarcoding for Characterizing the Microbiome and Salmonella Contamination of Retail Poultry Meat. J Food Prot. 2025 Feb 03; 88(2):100434.
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stilPCR increases the effective sequencing length of Illumina targeted next-generation sequencing. PLoS One. 2024; 19(12):e0314265.
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Comparison of Plasma Metagenomic Next-generation Sequencing and PCR Methods for Epstein-Barr Virus Viral Load Monitoring in Nasopharyngeal Carcinoma. Anticancer Res. 2024 Dec; 44(12):5445-5453.
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Digital PCR in Virology: Current Applications and Future Perspectives. Mol Diagn Ther. 2025 Jan; 29(1):43-54.
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Mouse Models for the Study of Borrelia burgdorferi Infection. Curr Protoc. 2024 Aug; 4(8):e1127.
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Assessing the Sensitivity of Nested PCR Followed by Direct Sequencing on Exosomal DNA for EGFR Mutation Detection in NSCL Iran Biomed J. 2024 07 01; 28(4):208-15.
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A CASE OF HERPES SIMPLEX VIRUS AND CYTOMEGALOVIRUS RETINITIS COINFECTION. Retin Cases Brief Rep. 2024 Jul 01; 18(4):451-454.
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Effects of fixation and demineralization on histomorphology and DNA amplification of canine bone marrow. Vet Pathol. 2024 11; 61(6):943-951.